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Category: Company News
Date: 2015-11-28
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100bp DNA Ladder H3 RTU 
Description 
A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. 
The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. 
The approximate mass of DNA in each band is provided (0.54 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. 

Source 
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCI(pH 8.0)and 10 mM EDTA 
Range:100-3,000 bp 
Number of bands:12 
Concentration:108 μg/ml 
Recommended Load:5 μl / well 
Containing orange G & xylene cyanol FF as tracking dyes. 
Storage 
Store at 25°C for 6 months 
Store at 4°C for 12months 
Store at -20°C for 24 months

1541211158796093526.jpg

1Kb DNA Ladder RTU 
Description 
A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. 
The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. 

Source 
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCI(pH 8.0)and 10 mM EDTA 

Range:250-10,000 bp 
Number of bands:13 
Concentration:100 μg/ml 
Package:50ug/500ul 
Recommended Load:5 μl / well 
Containing bromophenol blue & xylene cyanol FF as tracking dyes. 

Storage 
Store at 25°C for 6 months 
Store at 4°C for 12months 
Store at -20°C for 24 months

1541211184385082199.jpg


• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively• 2~3 μl per well for general Western transfering. 
• Apply more for thicker (>1.5 mm) or larger gel. 

Description 
The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. 
Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 
The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon , or nitrocellulose) and for approximate sizing of proteins. 
The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. 

Contents 
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) glycerol). 
Quality Control 
5 μl of BlueRAY Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. 
Storage 
Stable for up to 2 weeks at 25°C 
Stable for up to 3 months at 4°C 
For long term storage, store at -20°C 
Guide for Molecular Weight Estimation (kDa) 
Migration patterns of BLUeye Prestained Protein Ladder in different electrophoresis conditions are listed below:

1541211207542076654.jpg


Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition 
Supplement data should be considered for more accurate adjustment\ 
All products are for research use only. Caution: Not intended for human or animal diagnostic or therapeutic uses. 

BLUeye Prestained Protein Ladder 
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 
• 1.5~2.5 μl per well for general Western transferring. 
• Apply more for thicker (> 1.5 mm) or larger gel. 

Description 
The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. 
Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 
The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. 

Contents 
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). 

Quality Control 
Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane. 

Storage 
Stable for up to 2 weeks at 25°C 
Stable for up to 3 months at 4°C 
For long term storage, store at -20°C 
Guide for Molecular Weight Estimation (kDa) 
Migration patterns of BLUeye Prestained Protein Ladder in different electrophoresis conditions are listed below:

1541211231752018736.jpg

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